CNC

Stem cell expansion culture with minimal manipulation.

Heeyoung Lee. M.D., Hyunjin Yang. M.D.,

Baroyl Plastic Surgery., Medikan.Co., Seoul, Korea.

 Expansion of cell number would be the most important thing for the success of human cell therapy. During this cell expansion, trypsin dissolution is still necessary to move cells to new attachment surfaces. However, this trypsin is suspicious as a strong irritating chemical and mutagen to the cells, and it is still one of the biggest variable to decide the possibility of genetic transforms. More over the toxicity, repeated washing and centrifugation, reattachment would make apoxic period for 20 minutes. These conventional trypsin cultures could not be physiologic situations. 

 We used mechanical scraping in water proof vessels having multiple rubber ports would be good for trypsinless cell expansion with easy monitoring because we can not only see colony patterns but also fit medical protocols to prevent contaminations. We expanded the ASCs number 32 times within 3 weeks without trypsin treatment. For the movement of cells to new surfaces, we just used scraping with increasing the vessel numbers. When we need the cells for therapy, we scraped cells mechanically and use directly to patients. As results, we completely removed the trypsin over 200 times expansion of cell numbers, also we reduced whole manual processing hours under 20%.